Inhibition of lymphocyte proliferation by detergent-solubilized mouse liver membranes.
نویسندگان
چکیده
منابع مشابه
Inhibition of lymphocyte proliferation by detergent-solubilized mouse liver membranes
The role of phenomena analogous to fibroblast contact inhibition in lymphocyte growth regulation is controversial, although it is clear that direct cell-cell contact is vital to immunoregulation and accessory cell function. An extract of mouse liver plasma membrane proteins, referred to as suppressive liver extract (SLE), that suppresses the growth of 3T3 fibroblasts also inhibited the mitogen-...
متن کاملIsolation of the insulin receptor of liver and fat-cell membranes (detergent-solubilized-( 125 I)insulin-polyethylene glycol precipitation-sephadex).
Extraction of liver and fat-cell membranes with the nonionic detergent Triton X-100 prevents specific binding of (125)I-labeled insulin to these membranes. This loss of binding to particulate material is quantitatively recovered in a high-speed (300,000 x g, 2 hr) supernatant of the extract. Specific and reversible insulin binding to soluble proteins is readily demonstrable by gel filtration. A...
متن کاملStabilization of detergent-solubilized Ca2+-ATPase by poly(ethylene glycol).
The (Ca2+ + Mg2+)-ATPase from sarcoplasmic reticulum (SR) has been solubilized with 1-alkanoyl propanediol-3-phosphorylcholines with chainlengths ranging between 8 and 12 C atoms. A marked dependence of the ATPase activity upon the chainlength was found, indicating that alkyl chainlengths with 12 C atoms are necessary for retention of activity. Addition of poly(ethylene glycol) to the eluting b...
متن کاملp27Kip1 localizes to detergent-insoluble microdomains within lymphocyte membranes.
BACKGROUND Low levels of the cyclin-dependent kinase inhibitor p27Kip1 are associated with poor prognosis in cancer. It is unclear whether this is related strictly to p27Kip1-mediated cell cycle inhibition or to other, possibly extranuclear, roles of this protein. In this study, we examined p27Kip1 expression in quiescent and activated lymphocytes. T-cell membranes have been shown to possess sp...
متن کاملBinding properties of detergent-solubilized NCAM
An assay has been designed for the identification of NCAM-binding proteins present in an NP-40 detergent extract of brain membranes. This method, which is capable of analyzing both heterophilic and homophilic interactions, uses species-specific antibodies against NCAM in combination with radioiodination, so that after unlabeled chicken and iodinated frog brain membrane proteins were allowed to ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Cell Biology
سال: 1985
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.101.2.380